Composite

Part:BBa_K5276008

Designed by: Shuai Qin   Group: iGEM24_UCAS-China   (2024-09-26)


XylO

This system was constructed by combining the xylose repressor gene xylR, its operator sequence xylO, and the strong constitutive promoter PxylAB, the divergent promoter to drive the expression of the downstream gene. The XylR is expressed constitutively from PxylR. It binds to the XylR operator and blocks downstream gene expression from the PxylAB promoter without xylose. XylR is released from the operator with xylose, initiating the downstream gene expression.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None